Anti-Collagen I antibody (ab34710) | Abcam (2024)

Overview

• Product name

  Anti-Collagen I antibody
  See all Collagen I primary antibodies

• Description

  Rabbit polyclonal to Collagen I

• Host species

  Rabbit

• Specificity

  This product is not recommended for use under denaturing conditions in WB. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins.

  ab34710 has <5% cross-reactivity with Collagen III

  Customers have been successful using ab34710 in this application, please see reference Tillgren V et al. J Biol Chem 290:918-25; 2015.

  Positive Control: Human stomach, skin and adrenal gland tissue lysates.

• Tested applications

  Suitable for: IHC-P, WB, ICC/IFmore details

• Species reactivity

  Reacts with: Human
  

  See Also

  Renal antiporter ClC-5 regulates collagen I/IV through the {beta}-catenin pathway and lysosomal degradationThe KMT2A/MLL consensus gene structure: a comprehensive update for research and diagnostic implicationsAfrican Americans with translocation t(11;14) have superior survival after autologous hematopoietic cell transplantation for multiple myeloma compared in Whites in the United States

• Immunogen

  This product was produced with the following immunogens:
  Full length native protein (purified) corresponding to Human Collagen I aa 1-1400. Total Collagen Type I from human and bovine placenta
  Database link: P08123

  Full length native protein (purified) corresponding to Human Collagen I aa 1-1500. Total Collagen Type I from human and bovine placenta
  Database link: P02452

  Full length native protein (purified) corresponding to Bovine Collagen I aa 1-1400. Total Collagen Type I from human and bovine placenta
  Database link: P02465

  Full length native protein (purified) corresponding to Bovine Collagen I aa 1-1500. Total Collagen Type I from human and bovine placenta
  Database link: P02453

• Positive control

  • WB: human collagen.IHC-P: human stomach mucosa, smooth muscle cells of the human stomach wall and human tubuli and blood vessels.

• General notes

  Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.

  This collagen antibody was developed usingnon-denatured 3Depitopes, you must be careful not to denature the collagen protein during your experiment.

  PLEASE READ THESE IMPORTANT PROTOCOL TIPS, clickherefor the english version orherefor the mandarin version.

  It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues.

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.

• Storage buffer

  Preservative: 0.01% Sodium azide
  Constituents: 0.8766% Sodium chloride, 0.42% Potassium phosphate

• Concentration information loading...

• Purity

  Immunogen affinity purified

• Purification notes

  ab34710 has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered.

• Clonality

  Polyclonal

• Isotype

  IgG

• Research areas

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

Associated products

• Compatible Secondaries

• Isotype control

  • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

• Recombinant Protein

  • Recombinant Human Collagen I protein (ab158152)

• Related Products

  • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
  • Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
  • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
  • Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216791)
  • Native Cow Collagen I protein (ab7526)
  • Native Human Collagen I protein (ab7533)
  • Anti-Laminin 5 antibody [P3H9-2] (ab78286)
  • Anti-MMP2 antibody [6E3F8] (ab86607)

Applications

Target

• Function

  Type I collagen is a member of group I collagen (fibrillar forming collagen).

• Tissue specificity

  Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

• Involvement in disease

  Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
  Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
  Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
  Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
  Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.

• Sequence similarities

  Belongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.

• Post-translational
  modifications

  Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

• Cellular localization

  Secreted > extracellular space > extracellular matrix.

• Information by UniProt

• Database links

  • Entrez Gene: 1277 Human
  • Entrez Gene: 1278 Human
  • Omim: 120150 Human
  • Omim: 120160 Human
  • SwissProt: P02452 Human
  • SwissProt: P08123 Human
  • Unigene: 172928 Human
  • Unigene: 681002 Human

• Alternative names

  • Alpha 1 type I collagen antibody
  • Alpha 2 type I collagen antibody
  • alpha 2 type I procollagen antibody

  see all

Images

Protocols

Datasheets and documents

References (1659)